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The "B" series gene has some effect on the color of the nose, eye rims, lips, as well as the iris (color) of the eye. The "B" series only effects the eumelanin pigment. The eye color for the black dog ranges from yellowish to dark brown.
"B/B" or "B/b" allows the production of black pigment,
whereas "b/b" produces brown pigment wherever the dog would otherwise have produced black. The eye color of the brown dog ranges from greenish to amber to orange to brown.
A dog that is brown (chocolate) will have the genotype of "b/b D/D" or "b/b D/d" and will have a brown (chocolate) nose (because the "B" gene is not present and the "D" gene is in the dominant form).
If the "D" gene is in the recessive form ("d"), along with "b/b", then the nose would be a rosey-gray color --- like that of a fawn colored dog.
The "D", or dilution series gene, also has some effect on the color of the nose, eye rims, lips, as well as the iris of the eye. Eye color can range from very pale to light brown.
"D" is dominant and allows full pigmentation, whereas "d" is recessive and dilutes the pigment. The "D" series, in the homozygous recessive form ("d") effects both eumelanin and phaeomelanin, (in theory) by causing the clumping of the pigment granules in the hair. A dog that is dilute - black (called blue in the Kelpie breed) will have the genotype of "B/B d/d" or "B/b d/d" and will have a gray nose (because the "B" gene, which would normally cause the nose to be black, is now diluted to gray).
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